Please use this identifier to cite or link to this item:
|Title:||Oxytocinase activity in human spermatozoa and its possible role in fertilization||Authors:||Roy, A.C.
|Issue Date:||1990||Citation:||Roy, A.C., Ratnam, S.S. (1990). Oxytocinase activity in human spermatozoa and its possible role in fertilization. Archives of Andrology 25 (1) : 1-4. ScholarBank@NUS Repository.||Abstract:||Human spermatozoa were extracted with 1% Triton X-100 and analyzed for oxytocinase (E.C. 184.108.40.206) activity by means of two synthetic peptides, S-benzyl-L-cysteine-p-nitroanilide (BCN) and L-leucine-p-nitroanilide (LN), separately as substrates. The specific activity (mean ± SD) of this proteolytic enzyme at Vmax in eight different extracts was 0.150 ± 0.072 and 1.392 ± 0.602 mIU/106 cells using BCN and LN, respectively. The enzyme showed optimal activity at pH 7.2 when BCN was the substrate, and at pH 7.4 with LN as the substrate. Spermatozoal oxytocinase activity may be involved directly or indirectly in the reproductive mechanisms leading to sperm acrosome reaction and fertilization.||Source Title:||Archives of Andrology||URI:||http://scholarbank.nus.edu.sg/handle/10635/132586||ISSN:||01485016|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 16, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.