Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/132586
Title: Oxytocinase activity in human spermatozoa and its possible role in fertilization
Authors: Roy, A.C. 
Ratnam, S.S. 
Keywords: acrosome reaction
cystine aminopeptidase
fertilization
met-enkephalin
oxytocin
oxytocinase
spermatozoa
triton X-100
vasopressin
Issue Date: 1990
Source: Roy, A.C., Ratnam, S.S. (1990). Oxytocinase activity in human spermatozoa and its possible role in fertilization. Archives of Andrology 25 (1) : 1-4. ScholarBank@NUS Repository.
Abstract: Human spermatozoa were extracted with 1% Triton X-100 and analyzed for oxytocinase (E.C. 3.4.11.3) activity by means of two synthetic peptides, S-benzyl-L-cysteine-p-nitroanilide (BCN) and L-leucine-p-nitroanilide (LN), separately as substrates. The specific activity (mean ± SD) of this proteolytic enzyme at Vmax in eight different extracts was 0.150 ± 0.072 and 1.392 ± 0.602 mIU/106 cells using BCN and LN, respectively. The enzyme showed optimal activity at pH 7.2 when BCN was the substrate, and at pH 7.4 with LN as the substrate. Spermatozoal oxytocinase activity may be involved directly or indirectly in the reproductive mechanisms leading to sperm acrosome reaction and fertilization.
Source Title: Archives of Andrology
URI: http://scholarbank.nus.edu.sg/handle/10635/132586
ISSN: 01485016
Appears in Collections:Staff Publications

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