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|Title:||Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray||Authors:||Khadijah, S.
|Issue Date:||Sep-2003||Citation:||Khadijah, S., Neo, S.Y., Hossain, M.S., Miller, L.D., Mathavan, S., Kwang, J. (2003-09). Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray. Journal of Virology 77 (18) : 10162-10167. ScholarBank@NUS Repository. https://doi.org/10.1128/JVI.77.18.10162-10167.2003||Abstract:||To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.||Source Title:||Journal of Virology||URI:||http://scholarbank.nus.edu.sg/handle/10635/131490||ISSN:||0022538X||DOI:||10.1128/JVI.77.18.10162-10167.2003|
|Appears in Collections:||Staff Publications|
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