Please use this identifier to cite or link to this item: https://doi.org/10.1128/JVI.77.18.10162-10167.2003
Title: Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray
Authors: Khadijah, S.
Neo, S.Y. 
Hossain, M.S.
Miller, L.D. 
Mathavan, S. 
Kwang, J. 
Issue Date: Sep-2003
Citation: Khadijah, S., Neo, S.Y., Hossain, M.S., Miller, L.D., Mathavan, S., Kwang, J. (2003-09). Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray. Journal of Virology 77 (18) : 10162-10167. ScholarBank@NUS Repository. https://doi.org/10.1128/JVI.77.18.10162-10167.2003
Abstract: To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.
Source Title: Journal of Virology
URI: http://scholarbank.nus.edu.sg/handle/10635/131490
ISSN: 0022538X
DOI: 10.1128/JVI.77.18.10162-10167.2003
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