Please use this identifier to cite or link to this item:
|Title:||Induction of acute graft-versus-host disease by T cells that do not respond to in vitro alloantigen stimulation||Authors:||New, J.Y.
|Issue Date:||Sep-2004||Citation:||New, J.Y., Chan, S.H., Yap, E.H., Hu, H. (2004-09). Induction of acute graft-versus-host disease by T cells that do not respond to in vitro alloantigen stimulation. British Journal of Haematology 126 (6) : 828-836. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1365-2141.2004.05131.x||Abstract:||The mixed lymphocyte reaction (MLR) has been used extensively to measure alloreactive T cells. In clinical practice, a negative MLR of recipient T cells responding to donor cells does not necessarily mean that a donor-specific tolerance has been established. This discrepancy indicates that the presently used methods fail to demonstrate the full repertoire of alloreactive T cells. This could be the result of the fact that some alloreactive T cells do not respond in vitro but will mount a response towards alloantigens in vivo, or that some alloreactive T cells do not respond during the MLR but will respond later if the alloantigen stimulation remains. We therefore examined the non-proliferating T-cell population in a mouse primary alloreactive response. Spleen and lymph node cells derived from C57BL/6J (H-2 b) mice were stained with carboxy-fluorescein diacetate succinimidyl ester and injected intravenously into C.B-17 severe combined immunodeficient (SCID) mice (H-2 d). The donor cells were recovered 5 d after the injection. The non-proliferating T cells were sorted and were non-reactive to alloantigen stimulation in vitro. Nevertheless, these non-proliferating T cells could proliferate and cause acute graft-versus-host disease after being adoptively transferred to secondary recipients of SCID mice. These results suggest that there exists an alloreactive T-cell population that does not respond to in vitro alloantigen stimulation but can mount a delayed alloresponse in vivo.||Source Title:||British Journal of Haematology||URI:||http://scholarbank.nus.edu.sg/handle/10635/131252||ISSN:||00071048||DOI:||10.1111/j.1365-2141.2004.05131.x|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 18, 2019
WEB OF SCIENCETM
checked on Oct 10, 2019
checked on Oct 11, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.