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Title: Induction of acute graft-versus-host disease by T cells that do not respond to in vitro alloantigen stimulation
Authors: New, J.Y. 
Chan, S.H. 
Yap, E.H. 
Hu, H.
Keywords: Alloreactive response
Cellular proliferation
Graft-versus-host disease
T lymphocytes
Transplant tolerance
Issue Date: Sep-2004
Citation: New, J.Y., Chan, S.H., Yap, E.H., Hu, H. (2004-09). Induction of acute graft-versus-host disease by T cells that do not respond to in vitro alloantigen stimulation. British Journal of Haematology 126 (6) : 828-836. ScholarBank@NUS Repository.
Abstract: The mixed lymphocyte reaction (MLR) has been used extensively to measure alloreactive T cells. In clinical practice, a negative MLR of recipient T cells responding to donor cells does not necessarily mean that a donor-specific tolerance has been established. This discrepancy indicates that the presently used methods fail to demonstrate the full repertoire of alloreactive T cells. This could be the result of the fact that some alloreactive T cells do not respond in vitro but will mount a response towards alloantigens in vivo, or that some alloreactive T cells do not respond during the MLR but will respond later if the alloantigen stimulation remains. We therefore examined the non-proliferating T-cell population in a mouse primary alloreactive response. Spleen and lymph node cells derived from C57BL/6J (H-2 b) mice were stained with carboxy-fluorescein diacetate succinimidyl ester and injected intravenously into C.B-17 severe combined immunodeficient (SCID) mice (H-2 d). The donor cells were recovered 5 d after the injection. The non-proliferating T cells were sorted and were non-reactive to alloantigen stimulation in vitro. Nevertheless, these non-proliferating T cells could proliferate and cause acute graft-versus-host disease after being adoptively transferred to secondary recipients of SCID mice. These results suggest that there exists an alloreactive T-cell population that does not respond to in vitro alloantigen stimulation but can mount a delayed alloresponse in vivo.
Source Title: British Journal of Haematology
ISSN: 00071048
DOI: 10.1111/j.1365-2141.2004.05131.x
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