Please use this identifier to cite or link to this item: https://doi.org/10.1111/j.1365-2141.2004.05131.x
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dc.titleInduction of acute graft-versus-host disease by T cells that do not respond to in vitro alloantigen stimulation
dc.contributor.authorNew, J.Y.
dc.contributor.authorChan, S.H.
dc.contributor.authorYap, E.H.
dc.contributor.authorHu, H.
dc.date.accessioned2016-11-28T10:18:01Z
dc.date.available2016-11-28T10:18:01Z
dc.date.issued2004-09
dc.identifier.citationNew, J.Y., Chan, S.H., Yap, E.H., Hu, H. (2004-09). Induction of acute graft-versus-host disease by T cells that do not respond to in vitro alloantigen stimulation. British Journal of Haematology 126 (6) : 828-836. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1365-2141.2004.05131.x
dc.identifier.issn00071048
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131252
dc.description.abstractThe mixed lymphocyte reaction (MLR) has been used extensively to measure alloreactive T cells. In clinical practice, a negative MLR of recipient T cells responding to donor cells does not necessarily mean that a donor-specific tolerance has been established. This discrepancy indicates that the presently used methods fail to demonstrate the full repertoire of alloreactive T cells. This could be the result of the fact that some alloreactive T cells do not respond in vitro but will mount a response towards alloantigens in vivo, or that some alloreactive T cells do not respond during the MLR but will respond later if the alloantigen stimulation remains. We therefore examined the non-proliferating T-cell population in a mouse primary alloreactive response. Spleen and lymph node cells derived from C57BL/6J (H-2 b) mice were stained with carboxy-fluorescein diacetate succinimidyl ester and injected intravenously into C.B-17 severe combined immunodeficient (SCID) mice (H-2 d). The donor cells were recovered 5 d after the injection. The non-proliferating T cells were sorted and were non-reactive to alloantigen stimulation in vitro. Nevertheless, these non-proliferating T cells could proliferate and cause acute graft-versus-host disease after being adoptively transferred to secondary recipients of SCID mice. These results suggest that there exists an alloreactive T-cell population that does not respond to in vitro alloantigen stimulation but can mount a delayed alloresponse in vivo.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1111/j.1365-2141.2004.05131.x
dc.sourceScopus
dc.subjectAlloreactive response
dc.subjectCellular proliferation
dc.subjectGraft-versus-host disease
dc.subjectT lymphocytes
dc.subjectTransplant tolerance
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1111/j.1365-2141.2004.05131.x
dc.description.sourcetitleBritish Journal of Haematology
dc.description.volume126
dc.description.issue6
dc.description.page828-836
dc.description.codenBJHEA
dc.identifier.isiut000223895100009
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