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https://doi.org/10.1371/journal.pone.0016603
Title: | Rho GTPase Cdc42 is a direct interacting partner of adenomatous polyposis coli protein and can alter its cellular localization | Authors: | Sudhaharan, T. Goh, W.I. Sem, K.P. Lim, K.B. Bu, W. Ahmed, S. |
Issue Date: | 2011 | Citation: | Sudhaharan, T., Goh, W.I., Sem, K.P., Lim, K.B., Bu, W., Ahmed, S. (2011). Rho GTPase Cdc42 is a direct interacting partner of adenomatous polyposis coli protein and can alter its cellular localization. PLoS ONE 6 (2) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0016603 | Abstract: | Adenomatous Polyposis Coli (APC) is a tumor suppressor gene product involved in colon cancer. APC is a large multidomain molecule of 2843 amino acid residues and connects cell-cell adhesion, the F-actin/microtubule cytoskeleton and the nucleus. Here we show that Cdc42 interacts directly with the first three armadillo repeats of APC by yeast two-hybrid screens. We confirm the Cdc42-APC interaction using pulldown assays in vitro and FRET assays in vivo. Interestingly, Cdc42 interacts with APC at leading edge sites where F-actin is enriched. In contrast, Cdc42 interacts with the truncated mutant APC1-1638 in cellular puncta associated with the golgi-lysozome pathway in transfected CHO cells. In HCT116 and SW480 cells, Cdc42 induces the relocalization of endogenous APC and the mutant APC1-1338 to the plasma membrane and cellular puncta, respectively. Taken together, these data indicate that the Cdc42-APC interaction induces localization of both APC and mutant APC and may thus play a direct role in the functions of these proteins. © 2011 Sudhaharan et al. | Source Title: | PLoS ONE | URI: | http://scholarbank.nus.edu.sg/handle/10635/128864 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0016603 |
Appears in Collections: | Staff Publications |
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