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https://doi.org/10.1371/journal.pone.0016603
DC Field | Value | |
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dc.title | Rho GTPase Cdc42 is a direct interacting partner of adenomatous polyposis coli protein and can alter its cellular localization | |
dc.contributor.author | Sudhaharan, T. | |
dc.contributor.author | Goh, W.I. | |
dc.contributor.author | Sem, K.P. | |
dc.contributor.author | Lim, K.B. | |
dc.contributor.author | Bu, W. | |
dc.contributor.author | Ahmed, S. | |
dc.date.accessioned | 2016-10-22T07:45:12Z | |
dc.date.available | 2016-10-22T07:45:12Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Sudhaharan, T., Goh, W.I., Sem, K.P., Lim, K.B., Bu, W., Ahmed, S. (2011). Rho GTPase Cdc42 is a direct interacting partner of adenomatous polyposis coli protein and can alter its cellular localization. PLoS ONE 6 (2) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0016603 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/128864 | |
dc.description.abstract | Adenomatous Polyposis Coli (APC) is a tumor suppressor gene product involved in colon cancer. APC is a large multidomain molecule of 2843 amino acid residues and connects cell-cell adhesion, the F-actin/microtubule cytoskeleton and the nucleus. Here we show that Cdc42 interacts directly with the first three armadillo repeats of APC by yeast two-hybrid screens. We confirm the Cdc42-APC interaction using pulldown assays in vitro and FRET assays in vivo. Interestingly, Cdc42 interacts with APC at leading edge sites where F-actin is enriched. In contrast, Cdc42 interacts with the truncated mutant APC1-1638 in cellular puncta associated with the golgi-lysozome pathway in transfected CHO cells. In HCT116 and SW480 cells, Cdc42 induces the relocalization of endogenous APC and the mutant APC1-1338 to the plasma membrane and cellular puncta, respectively. Taken together, these data indicate that the Cdc42-APC interaction induces localization of both APC and mutant APC and may thus play a direct role in the functions of these proteins. © 2011 Sudhaharan et al. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.pone.0016603 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE | |
dc.description.doi | 10.1371/journal.pone.0016603 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 6 | |
dc.description.issue | 2 | |
dc.description.page | - | |
dc.identifier.isiut | 000287036200022 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications |
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10_1371_journal_pone_0016603.pdf | 1.67 MB | Adobe PDF | OPEN | Published | View/Download |
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