Please use this identifier to cite or link to this item: https://doi.org/10.1073/pnas.1301766110
Title: Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis
Authors: Masters, T.A.
Pontes, B.
Viasnoff, V. 
Li, Y.
Gauthier, N.C. 
Keywords: Frustrated phagocytosis
Immunology
Phagocytic cell
Rho GTPases
Traffic
Issue Date: 16-Jul-2013
Citation: Masters, T.A., Pontes, B., Viasnoff, V., Li, Y., Gauthier, N.C. (2013-07-16). Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis. Proceedings of the National Academy of Sciences of the United States of America 110 (29) : 11875-11880. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.1301766110
Abstract: Phagocytes clear the body of undesirable particles such as infectious agents and debris. To extend pseudopods over the surface of targeted particles during engulfment, cells must change shape through extensive membrane and cytoskeleton remodeling. We observed that pseudopod extension occurred in two phases. In the first phase, pseudopods extended rapidly, with actin polymerization pushing the plasma membrane forward. The second phase occurred once the membrane area from preexisting reservoirs was depleted, leading to increased membrane tension. Increased tension directly altered the small Rho GTPase Rac1, 3'-phosphoinositide, and cytoskeletal organization. Furthermore, it activated exocytosis of vesicles containing GPI-Anchored proteins, increasing membrane area and phagocytosis efficiency for large particles. We thus propose that, during phagocytosis, membrane remodeling, cytoskeletal organization, and biochemical signaling are orchestrated by the mechanical signal of membrane tension. These results put a simple mechanical signal at the heart of understanding immunological responses.
Source Title: Proceedings of the National Academy of Sciences of the United States of America
URI: http://scholarbank.nus.edu.sg/handle/10635/126674
ISSN: 00278424
DOI: 10.1073/pnas.1301766110
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

89
checked on Dec 1, 2020

WEB OF SCIENCETM
Citations

81
checked on Dec 1, 2020

Page view(s)

50
checked on Nov 28, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.