Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/120748
Title: Hydroxyl free radicals generated by vanadyl[IV] induce cell blebbing in mitotic human Chang liver cells
Authors: Bay, B.-H. 
Sit, K.-H. 
Paramanantham, R. 
Chan, Y.-G.
Keywords: Confocal microscopy
Glutathione
Hydroxyl free radicals
Scanning electron microscopy
Vanadium
Issue Date: 1997
Citation: Bay, B.-H.,Sit, K.-H.,Paramanantham, R.,Chan, Y.-G. (1997). Hydroxyl free radicals generated by vanadyl[IV] induce cell blebbing in mitotic human Chang liver cells. BioMetals 10 (2) : 119-122. ScholarBank@NUS Repository.
Abstract: Vanadium has recently been reported to induce interphase and M-phase (mitotic) programmed cell death via the generation of hydroxyl free radicals (OH(*)). In this paper, the effects of antioxidants on: (a) vanadyl[IV]-generated OH(*) free radical levels; and (b) cellular glutathione in vanadyl [IV]-treated Chang liver cells were evaluated. The surface morphology of vanadyl-treated mitotic cells was studied by confocal and scanning microscopy. The free radical scavengers zinc chloride, glucose and thiourea reduced the levels of vanadyl-induced OH(*) free radicals and partially prevented the depletion of cellular glutathione. Concurrent with OH(*) free radical production, vanadyl-treated telophase cells exhibited excessive cell blebbing and cell shrinkage. The morphological features demonstrated in vanadyl-induced mitotic programmed cell death as a consequence of oxidative stress is novel.
Source Title: BioMetals
URI: http://scholarbank.nus.edu.sg/handle/10635/120748
ISSN: 09660844
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

27
checked on Aug 16, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.