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Title: Azide- and vanadate-sensitive M-phase alkalinity and cytosolic acidification of Chang liver cells
Authors: Sit, K.-H. 
Paramanantham, R.
Bay, B.-H. 
Wong, K.-P.
Keywords: ATPase sensitivity (F-ATPase and P-ATPase)
Flow cytometric evaluation (BCECF ratio)
Proton pumping (M-phase and interphase)
Issue Date: 1994
Citation: Sit, K.-H.,Paramanantham, R.,Bay, B.-H.,Wong, K.-P. (1994). Azide- and vanadate-sensitive M-phase alkalinity and cytosolic acidification of Chang liver cells. Japanese Journal of Pharmacology 65 (1) : 83-87. ScholarBank@NUS Repository.
Abstract: Flow cytometric cell-by-cell evaluation of NH4Cl acidification of human Chang cells showed that at steady state, 3% of the cells remained alkalinized (> PH(i) 7) over an extended period (up to 80 min) despite the absence of extracellular Na+ and HCO3 -. In fluorescence microscopy, the acidification-resistant cells were characteristically rounded M-phase cells. Both mean cytosolic pH and M-phase alkalinity were however sensitive to (a) azide and oligomycin, inhibitors of F-ATPase (ATP synthase), and to (b) vanadium ions, the phosphate analogue of P-ATPase (ATP-hydrolyzing), in dose-dependent and time-dependent manners. Dead cell indices were constant at ≃ 10%. Thiocyanate chaotrophic anions, which cleave the V-ATPase structure, had no effect. Since ATP synthesizing F-ATPase (ATP synthase) is coupled to ATP-hydrolyzing P-ATPase as 'master-and-slave', azide- and oligomycin-sensitivity corroborated with vanadate-sensitivity in suggesting energized proton pumping modulating (a) M-phase alkalinity and (b) cytosolic pH, against acidification.
Source Title: Japanese Journal of Pharmacology
ISSN: 00215198
Appears in Collections:Staff Publications

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