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https://scholarbank.nus.edu.sg/handle/10635/119249
| Title: | Vector design for monoclonal antibody production using Chinese hamster ovary cells | Authors: | HO CHENG LEONG STEVEN | Keywords: | Chinese hamster ovary (CHO) cells, Monoclonal antibody (mAb), IRES, F2A, Glycosylation, Aggregation | Issue Date: | 1-Aug-2014 | Citation: | HO CHENG LEONG STEVEN (2014-08-01). Vector design for monoclonal antibody production using Chinese hamster ovary cells. ScholarBank@NUS Repository. | Abstract: | A plasmid vector utilizing IRES elements was developed for stable expression of monoclonal antibody in CHO cells. The vector reduced false positives, increased selection stringency and controlled light chain to heavy chain ratio at 3:1. The product collected was shown to have low aggregate levels and consistent glycosylation. | URI: | http://scholarbank.nus.edu.sg/handle/10635/119249 |
| Appears in Collections: | Ph.D Theses (Open) |
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| File | Description | Size | Format | Access Settings | Version | |
|---|---|---|---|---|---|---|
| HoCLS.pdf | 3.93 MB | Adobe PDF | OPEN | None | View/Download |
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