Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/119249
Title: Vector design for monoclonal antibody production using Chinese hamster ovary cells
Authors: HO CHENG LEONG STEVEN
Keywords: Chinese hamster ovary (CHO) cells, Monoclonal antibody (mAb), IRES, F2A, Glycosylation, Aggregation
Issue Date: 1-Aug-2014
Source: HO CHENG LEONG STEVEN (2014-08-01). Vector design for monoclonal antibody production using Chinese hamster ovary cells. ScholarBank@NUS Repository.
Abstract: A plasmid vector utilizing IRES elements was developed for stable expression of monoclonal antibody in CHO cells. The vector reduced false positives, increased selection stringency and controlled light chain to heavy chain ratio at 3:1. The product collected was shown to have low aggregate levels and consistent glycosylation.
URI: http://scholarbank.nus.edu.sg/handle/10635/119249
Appears in Collections:Ph.D Theses (Open)

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