Vector design for monoclonal antibody production using Chinese hamster ovary cells

Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/119249

DC Field	Value
dc.title	Vector design for monoclonal antibody production using Chinese hamster ovary cells
dc.contributor.author	HO CHENG LEONG STEVEN
dc.date.accessioned	2015-03-31T18:01:06Z
dc.date.available	2015-03-31T18:01:06Z
dc.date.issued	2014-08-01
dc.identifier.citation	HO CHENG LEONG STEVEN (2014-08-01). Vector design for monoclonal antibody production using Chinese hamster ovary cells. ScholarBank@NUS Repository.
dc.identifier.uri	http://scholarbank.nus.edu.sg/handle/10635/119249
dc.description.abstract	A plasmid vector utilizing IRES elements was developed for stable expression of monoclonal antibody in CHO cells. The vector reduced false positives, increased selection stringency and controlled light chain to heavy chain ratio at 3:1. The product collected was shown to have low aggregate levels and consistent glycosylation.
dc.language.iso	en
dc.subject	Chinese hamster ovary (CHO) cells, Monoclonal antibody (mAb), IRES, F2A, Glycosylation, Aggregation
dc.type	Thesis
dc.contributor.department	BIOMEDICAL ENGINEERING
dc.contributor.supervisor	TONG YEN WAH
dc.contributor.supervisor	YANG YUANSHENG
dc.description.degree	Ph.D
dc.description.degreeconferred	DOCTOR OF PHILOSOPHY
dc.identifier.isiut	NOT_IN_WOS
Appears in Collections:	Ph.D Theses (Open)

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