Please use this identifier to cite or link to this item: https://doi.org/10.1002/mc.21951
Title: The topography of DNA methylation in the non-neoplastic colonic mucosa surrounding colorectal cancers
Authors: Subramaniam, M.M. 
Loh, M.
Chan, J.Y.
Liem, N.
Lim, P.L.
Peng, Y.W. 
Lim, X.Y. 
Yeoh, K.G.
Iacopetta, B.
Soong, R.
Salto-Tellez, M. 
Keywords: Colorectal cancer
Colorectal field
DNA methylation
Field effect
Non-neoplastic mucosa
Issue Date: Feb-2014
Citation: Subramaniam, M.M., Loh, M., Chan, J.Y., Liem, N., Lim, P.L., Peng, Y.W., Lim, X.Y., Yeoh, K.G., Iacopetta, B., Soong, R., Salto-Tellez, M. (2014-02). The topography of DNA methylation in the non-neoplastic colonic mucosa surrounding colorectal cancers. Molecular Carcinogenesis 53 (2) : 98-108. ScholarBank@NUS Repository. https://doi.org/10.1002/mc.21951
Abstract: The degree of gene hypermethylation in non-neoplastic colonic mucosa (NNCM) is a potentially important event in the development of colorectal cancer (CRC), particularly for the subgroup with a CpG island methylator phenotype (CIMP). In this study, we aimed to use an unbiased and high-throughput approach to evaluate the topography of DNA methylation in the non-neoplastic colonic mucosa (NNCM) surrounding colorectal cancer (CRC). A total of 61 tissue samples comprising 53 NNCM and 8 tumor samples were obtained from hemicolectomy specimens of two CRC patients (Cases 1 and 2). NNCM was stripped from the underlying colonic wall and samples taken at varying distances from the tumor. The level of DNA methylation in NNCM and tumor tissues was assessed at 1,505 CpG sites in 807 cancer-related genes using Illumina GoldenGate® methylation arrays. Case 1 tumor showed significantly higher levels of methylation compared to surrounding NNCM samples (P
Source Title: Molecular Carcinogenesis
URI: http://scholarbank.nus.edu.sg/handle/10635/116648
ISSN: 08991987
DOI: 10.1002/mc.21951
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