Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1002678
Title: SPE-44 implements sperm cell fate
Authors: Kulkarni, M. 
Shakes, D.C.
Guevel, K.
Smith, H.E.
Issue Date: Apr-2012
Citation: Kulkarni, M., Shakes, D.C., Guevel, K., Smith, H.E. (2012-04). SPE-44 implements sperm cell fate. PLoS Genetics 8 (4) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1002678
Abstract: The sperm/oocyte decision in the hermaphrodite germline of Caenorhabditis elegans provides a powerful model for the characterization of stem cell fate specification and differentiation. The germline sex determination program that governs gamete fate has been well studied, but direct mediators of cell-type-specific transcription are largely unknown. We report the identification of spe-44 as a critical regulator of sperm gene expression. Deletion of spe-44 causes sperm-specific defects in cytokinesis, cell cycle progression, and organelle assembly resulting in sterility. Expression of spe-44 correlates precisely with spermatogenesis and is regulated by the germline sex determination pathway. spe-44 is required for the appropriate expression of several hundred sperm-enriched genes. The SPE-44 protein is restricted to the sperm-producing germline, where it localizes to the autosomes (which contain sperm genes) but is excluded from the transcriptionally silent X chromosome (which does not). The orthologous gene in other Caenorhabditis species is similarly expressed in a sex-biased manner, and the protein likewise exhibits autosome-specific localization in developing sperm, strongly suggestive of an evolutionarily conserved role in sperm gene expression. Our analysis represents the first identification of a transcriptional regulator whose primary function is the control of gamete-type-specific transcription in this system.
Source Title: PLoS Genetics
URI: http://scholarbank.nus.edu.sg/handle/10635/116601
ISSN: 15537390
DOI: 10.1371/journal.pgen.1002678
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