Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1002678
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dc.titleSPE-44 implements sperm cell fate
dc.contributor.authorKulkarni, M.
dc.contributor.authorShakes, D.C.
dc.contributor.authorGuevel, K.
dc.contributor.authorSmith, H.E.
dc.date.accessioned2014-12-12T07:51:53Z
dc.date.available2014-12-12T07:51:53Z
dc.date.issued2012-04
dc.identifier.citationKulkarni, M., Shakes, D.C., Guevel, K., Smith, H.E. (2012-04). SPE-44 implements sperm cell fate. PLoS Genetics 8 (4) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1002678
dc.identifier.issn15537390
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/116601
dc.description.abstractThe sperm/oocyte decision in the hermaphrodite germline of Caenorhabditis elegans provides a powerful model for the characterization of stem cell fate specification and differentiation. The germline sex determination program that governs gamete fate has been well studied, but direct mediators of cell-type-specific transcription are largely unknown. We report the identification of spe-44 as a critical regulator of sperm gene expression. Deletion of spe-44 causes sperm-specific defects in cytokinesis, cell cycle progression, and organelle assembly resulting in sterility. Expression of spe-44 correlates precisely with spermatogenesis and is regulated by the germline sex determination pathway. spe-44 is required for the appropriate expression of several hundred sperm-enriched genes. The SPE-44 protein is restricted to the sperm-producing germline, where it localizes to the autosomes (which contain sperm genes) but is excluded from the transcriptionally silent X chromosome (which does not). The orthologous gene in other Caenorhabditis species is similarly expressed in a sex-biased manner, and the protein likewise exhibits autosome-specific localization in developing sperm, strongly suggestive of an evolutionarily conserved role in sperm gene expression. Our analysis represents the first identification of a transcriptional regulator whose primary function is the control of gamete-type-specific transcription in this system.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.pgen.1002678
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.description.doi10.1371/journal.pgen.1002678
dc.description.sourcetitlePLoS Genetics
dc.description.volume8
dc.description.issue4
dc.description.page-
dc.identifier.isiut000303441800044
dc.published.statePublished
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