Please use this identifier to cite or link to this item:
|Title:||A positive feedback loop between dumbfounded and rolling pebbles leads to myotube enlargement in Drosophila||Authors:||Menon, S.D.
|Issue Date:||Jun-2005||Citation:||Menon, S.D., Osman, Z., Chenchill, K., Chia, W. (2005-06). A positive feedback loop between dumbfounded and rolling pebbles leads to myotube enlargement in Drosophila. Journal of Cell Biology 169 (6) : 909-920. ScholarBank@NUS Repository. https://doi.org/10.1083/jcb.200501126||Abstract:||In Drosophila, myoblasts are subdivided into founders and fusion-competent myoblasts (fcm) with myotubes forming through fusion of one founder and several fcm. Duf and rolling pebbles 7 (Rols7; also known as antisocial) are expressed in founders, whereas sticks and stones (SNS) is present in fcm. Duf attracts fcm toward founders and also causes translocation of Rols7 from the cytoplasm to the fusion site. We show that Duf is a type 1 transmembrane protein that induces Rols7 translocation specifically when present intact and engaged in homophilic or Duf-SNS adhesion. Although its membrane-anchored extracellular domain functions as an attractant and is sufficient for the initial round of fusion, subsequent fusions require replenishment of Duf through cotranslocation with Rols7 tetratricopeptide repeat/coiled-coil domain-containing vesicles to the founder/myotube surface, causing both Duf and Rols7 to be at fusion sites between founders/myotubes and fcm. This implicates the Duf-Rols7 positive feedback loop to the occurrence of fusion at specific sites along the membrane and provides a mechanism by which the rate of fusion is controlled. © The Rockefeller University Press.||Source Title:||Journal of Cell Biology||URI:||http://scholarbank.nus.edu.sg/handle/10635/115567||ISSN:||00219525||DOI:||10.1083/jcb.200501126|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.