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https://doi.org/10.1016/j.febslet.2012.03.067
| Title: | α-Actinin4 nuclear translocation mediates gonadotropin-releasing hormone stimulation of follicle-stimulating hormone β-subunit gene transcription in LβT2 cells | Authors: | Yu, H. Li, Z. Ghosh, D. Lim, T.K. He, Y. Lin, Q. |
Keywords: | ACTN4 Calmodulin-like domain Fshβ GnRH Nuclear translocation |
Issue Date: | 21-May-2012 | Citation: | Yu, H., Li, Z., Ghosh, D., Lim, T.K., He, Y., Lin, Q. (2012-05-21). α-Actinin4 nuclear translocation mediates gonadotropin-releasing hormone stimulation of follicle-stimulating hormone β-subunit gene transcription in LβT2 cells. FEBS Letters 586 (10) : 1466-1471. ScholarBank@NUS Repository. https://doi.org/10.1016/j.febslet.2012.03.067 | Abstract: | Gonadotropin-releasing hormone (GnRH) regulates the synthesis and secretion of follicle-stimulating hormone (FSH) by stimulating the transcription of Fshβ gene. Our iTRAQ quantitative proteomics result showed that the abundance of α-actinin4 (ACTN4) increased in the nuclei of LβT2 cells upon GnRH induction. Using RNA interference, reverse transcription and real-time PCR, luciferase and transient transfection assays, we proved that ACTN4 is involved in the regulation of mouse Fshβ gene (mFshβ) transcription and its C-terminal calmodulin (CaM)-like domain is crucial for this process. Our study suggests that ACTN4 nuclear translocation mediates GnRH stimulation of mFshβ gene transcription. © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. | Source Title: | FEBS Letters | URI: | http://scholarbank.nus.edu.sg/handle/10635/115360 | ISSN: | 00145793 | DOI: | 10.1016/j.febslet.2012.03.067 |
| Appears in Collections: | Staff Publications |
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