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|Title:||Sepharose-supported DNA as template for RNA synthesis||Authors:||Cui, T.
|Keywords:||In vitro RNA synthesis
|Issue Date:||11-Dec-1998||Citation:||Cui, T., Porter, A.G., Gutte, B. (1998-12-11). Sepharose-supported DNA as template for RNA synthesis. Journal of Biotechnology 66 (2-3) : 225-228. ScholarBank@NUS Repository. https://doi.org/10.1016/S0168-1656(98)00145-X||Abstract:||A 96-bp DNA fragment containing the bacteriophage SP6 promoter was covalently coupled to EAH-Sepharose 4B in an orientation-specific manner. Here we show that the immobilized DNA was able to serve as template in in vitro transcription of RNA. This solid-phase approach allowed easy separation of the RNA products by centrifugation and made digestion of the template DNA by DNase superfluous. The Sepharose-bound DNA template was active in at least four consecutive cycles of in vitro transcription whose results were highly reproducible. The system may form a useful basis for the development of other molecular biology reactions on solid supports. Copyright (C) 1998 Elsevier Science B.V.||Source Title:||Journal of Biotechnology||URI:||http://scholarbank.nus.edu.sg/handle/10635/115283||ISSN:||01681656||DOI:||10.1016/S0168-1656(98)00145-X|
|Appears in Collections:||Staff Publications|
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