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Title: Sepharose-supported DNA as template for RNA synthesis
Authors: Cui, T. 
Porter, A.G. 
Gutte, B.
Keywords: In vitro RNA synthesis
Sepharose-supported DNA
Issue Date: 11-Dec-1998
Citation: Cui, T., Porter, A.G., Gutte, B. (1998-12-11). Sepharose-supported DNA as template for RNA synthesis. Journal of Biotechnology 66 (2-3) : 225-228. ScholarBank@NUS Repository.
Abstract: A 96-bp DNA fragment containing the bacteriophage SP6 promoter was covalently coupled to EAH-Sepharose 4B in an orientation-specific manner. Here we show that the immobilized DNA was able to serve as template in in vitro transcription of RNA. This solid-phase approach allowed easy separation of the RNA products by centrifugation and made digestion of the template DNA by DNase superfluous. The Sepharose-bound DNA template was active in at least four consecutive cycles of in vitro transcription whose results were highly reproducible. The system may form a useful basis for the development of other molecular biology reactions on solid supports. Copyright (C) 1998 Elsevier Science B.V.
Source Title: Journal of Biotechnology
ISSN: 01681656
DOI: 10.1016/S0168-1656(98)00145-X
Appears in Collections:Staff Publications

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