Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0168-1656(98)00145-X
DC FieldValue
dc.titleSepharose-supported DNA as template for RNA synthesis
dc.contributor.authorCui, T.
dc.contributor.authorPorter, A.G.
dc.contributor.authorGutte, B.
dc.date.accessioned2014-12-12T07:13:30Z
dc.date.available2014-12-12T07:13:30Z
dc.date.issued1998-12-11
dc.identifier.citationCui, T., Porter, A.G., Gutte, B. (1998-12-11). Sepharose-supported DNA as template for RNA synthesis. Journal of Biotechnology 66 (2-3) : 225-228. ScholarBank@NUS Repository. https://doi.org/10.1016/S0168-1656(98)00145-X
dc.identifier.issn01681656
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/115283
dc.description.abstractA 96-bp DNA fragment containing the bacteriophage SP6 promoter was covalently coupled to EAH-Sepharose 4B in an orientation-specific manner. Here we show that the immobilized DNA was able to serve as template in in vitro transcription of RNA. This solid-phase approach allowed easy separation of the RNA products by centrifugation and made digestion of the template DNA by DNase superfluous. The Sepharose-bound DNA template was active in at least four consecutive cycles of in vitro transcription whose results were highly reproducible. The system may form a useful basis for the development of other molecular biology reactions on solid supports. Copyright (C) 1998 Elsevier Science B.V.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0168-1656(98)00145-X
dc.sourceScopus
dc.subjectIn vitro RNA synthesis
dc.subjectSepharose-supported DNA
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.doi10.1016/S0168-1656(98)00145-X
dc.description.sourcetitleJournal of Biotechnology
dc.description.volume66
dc.description.issue2-3
dc.description.page225-228
dc.description.codenJBITD
dc.identifier.isiut000077604300016
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