Please use this identifier to cite or link to this item: https://doi.org/10.1002/path.1555
Title: Prion protein is ubiquitinated after developing protease resistance in the brains of scrapie-infected mice
Authors: Kang, S.-C.
Brown, D.R.
Whiteman, M.
Li, R.
Pan, T.
Perry, G.
Wisniewski, T.
Sy, M.-S.
Wong, B.-S. 
Keywords: ME7
Prion
Sandwich ELISA
Scrapie
Ubiquitin
Issue Date: May-2004
Citation: Kang, S.-C., Brown, D.R., Whiteman, M., Li, R., Pan, T., Perry, G., Wisniewski, T., Sy, M.-S., Wong, B.-S. (2004-05). Prion protein is ubiquitinated after developing protease resistance in the brains of scrapie-infected mice. Journal of Pathology 203 (1) : 603-608. ScholarBank@NUS Repository. https://doi.org/10.1002/path.1555
Abstract: Although the key event in the pathology of prion diseases is thought to be the conversion of cellular prion protein (PrPC) to the protease-resistant scrapie species termed PrPSc, the factors that contribute to neurodegeneration in scrapie-infected animals are poorly understood. One probable determinant could be when the accumulation of PrPSc in infected brain overwhelms the ubiquitin-proteasome system and triggers the degenerative cascade. In the present study, it was found that in mouse brains infected with the ME7 scrapie strain, the level of ubiquitin protein conjugates increased significantly at ∼144 days post-infection (pi) when clinical signs first become apparent. This elevation correlated with the detection of protease-resistant PrPSc and a decline in two endopeptidase activities associated with proteasome function. However, ubiquitination of PrP was only detected at the terminal stage, 3 weeks after the development of clinical symptoms (∼165 days pi). These results suggest that ubiquitination of PrP is a late event phenomenon and this conjugation occurs after the formation of protease-resistant PrPSc. Whether this post-translational modification and the impairment of proteasome function are pivotal events in the pathogenesis of prion diseases remains to be determined. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Source Title: Journal of Pathology
URI: http://scholarbank.nus.edu.sg/handle/10635/113609
ISSN: 00223417
DOI: 10.1002/path.1555
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