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Title: Genetic linkage maps of the guppy (Poecilia reticulata): Assignment of RAPD markers to multipoint linkage groups
Authors: Khoo, G. 
Lim, M.H.
Suresh, H.
Gan, D.K.Y.
Lim, K.F.
Chen, F.
Chan, W.-K. 
Lim, T.M. 
Phang, V.P.E. 
Keywords: Guppy
Linkage maps
Phenotypic markers
Poecilia reticulata
RAPD markers
Issue Date: May-2003
Citation: Khoo, G., Lim, M.H., Suresh, H., Gan, D.K.Y., Lim, K.F., Chen, F., Chan, W.-K., Lim, T.M., Phang, V.P.E. (2003-05). Genetic linkage maps of the guppy (Poecilia reticulata): Assignment of RAPD markers to multipoint linkage groups. Marine Biotechnology 5 (3) : 279-293. ScholarBank@NUS Repository.
Abstract: Genetic linkage maps of the guppy (Poecilia reticulata) were constructed from independent crosses between the Tuxedo strain and a feral line (Wildtype). Segregation patterns of random amplified polymorphic DNA (RAPD) markers and phenotypic markers were investigated in F2 offspring of Tuxedo ♂♂ x Wildtype♀♀ and Wildtype ♂♂ x Tuxedo ♀♀ crosses. Among the 300 and 276 RAPD markers scored for the respective crosses, linkages were identified for 230 and 212, respectively. The Tuxedo ♂♂ x Wildtype ♀♀ and Wildtype ♂♂ x Tuxedo ♀♀ maps spanned 2100 Kosambi centiMorgans (cMK) and 1900 CMK, respectively, in 28 linkage groups. Average marker resolution was 10 CMK. Genome length was estimated at 4410 CM K and 4060 CMK for the respective crosses, with an average physical distance of 166 kbp/cMK. Several RAPD markers were closely linked to or mapped onto the loci for the sex-determining region (SdR), and the sex-linked black caudal-peduncle (Bcp) and red tail (Rdt) genes. These primary linkage maps are the initial step toward the construction of a composite high-density map to facilitate map-based cloning and marker-assisted selection of quantitative trait loci that are essential for the development of comprehensive breeding programs for the guppy.
Source Title: Marine Biotechnology
ISSN: 14362228
DOI: 10.1007/s10126-002-0072-3
Appears in Collections:Staff Publications

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