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|Title:||Genetic linkage maps of the guppy (Poecilia reticulata): Assignment of RAPD markers to multipoint linkage groups||Authors:||Khoo, G.
|Issue Date:||May-2003||Citation:||Khoo, G., Lim, M.H., Suresh, H., Gan, D.K.Y., Lim, K.F., Chen, F., Chan, W.-K., Lim, T.M., Phang, V.P.E. (2003-05). Genetic linkage maps of the guppy (Poecilia reticulata): Assignment of RAPD markers to multipoint linkage groups. Marine Biotechnology 5 (3) : 279-293. ScholarBank@NUS Repository. https://doi.org/10.1007/s10126-002-0072-3||Abstract:||Genetic linkage maps of the guppy (Poecilia reticulata) were constructed from independent crosses between the Tuxedo strain and a feral line (Wildtype). Segregation patterns of random amplified polymorphic DNA (RAPD) markers and phenotypic markers were investigated in F2 offspring of Tuxedo ♂♂ x Wildtype♀♀ and Wildtype ♂♂ x Tuxedo ♀♀ crosses. Among the 300 and 276 RAPD markers scored for the respective crosses, linkages were identified for 230 and 212, respectively. The Tuxedo ♂♂ x Wildtype ♀♀ and Wildtype ♂♂ x Tuxedo ♀♀ maps spanned 2100 Kosambi centiMorgans (cMK) and 1900 CMK, respectively, in 28 linkage groups. Average marker resolution was 10 CMK. Genome length was estimated at 4410 CM K and 4060 CMK for the respective crosses, with an average physical distance of 166 kbp/cMK. Several RAPD markers were closely linked to or mapped onto the loci for the sex-determining region (SdR), and the sex-linked black caudal-peduncle (Bcp) and red tail (Rdt) genes. These primary linkage maps are the initial step toward the construction of a composite high-density map to facilitate map-based cloning and marker-assisted selection of quantitative trait loci that are essential for the development of comprehensive breeding programs for the guppy.||Source Title:||Marine Biotechnology||URI:||http://scholarbank.nus.edu.sg/handle/10635/113489||ISSN:||14362228||DOI:||10.1007/s10126-002-0072-3|
|Appears in Collections:||Staff Publications|
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