Please use this identifier to cite or link to this item:
Title: Calpain activation after mitochondrial permeability transition in microcystin-induced cell death in rat hepatocytes
Authors: Ding, W.-X.
Shen, H.-M. 
Ong, C.-N. 
Keywords: Apoptosis
Electron transport chain
Intracellular Ca2+
Oxidative stress
Issue Date: 2002
Citation: Ding, W.-X., Shen, H.-M., Ong, C.-N. (2002). Calpain activation after mitochondrial permeability transition in microcystin-induced cell death in rat hepatocytes. Biochemical and Biophysical Research Communications 291 (2) : 321-331. ScholarBank@NUS Repository.
Abstract: Previous studies have shown that microcystin-LR (MLR), a specific hepatotoxin, induces onset of mitochondrial permeability transition (MPT) and apoptosis in cultured rat hepatocytes. Here we attempted to investigate the downstream events after the onset of MPT in MLR-treated hepatocytes. Various mitochondrial electron transport chain (ETC) inhibitors effectively prevented the onset of MPT, suggesting that the mitochondrial ETC plays an important role in MLR-induced MPT. MLR also induced mitochondrial cytochrome c release, which can be prevented by a specific MPT inhibitor (cyclosporin A, CsA), and by various ETC inhibitors. Interestingly, the release of cytochrome c did not activate caspase-9 and -3, the main caspases involved in apoptosis. Instead, MLR activated calpain in rat hepatocytes, probably through the increase of intracellular Ca2+ released from mitochondria. Both ALLN and ALLM, two calpain inhibitors, significantly blocked MLR-induced calpain activation and subsequent cell death. CsA also prevented MLR-induced calpain activation and cell death, suggesting that the activation of calpain may be a post-mitochondrial event. These data demonstrate for the first time that calpain rather than caspases plays an important role in MLR-induced apoptosis. © 2002 Elsevier Science (USA).
Source Title: Biochemical and Biophysical Research Communications
ISSN: 0006291X
DOI: 10.1006/bbrc.2002.6453
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Oct 19, 2021


checked on Oct 19, 2021

Page view(s)

checked on Oct 14, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.