Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1017970030131
Title: Cadmium-induced apoptosis and phenotypic changes in mouse thymocytes
Authors: Dong, S.
Shen, H.-M. 
Ong, C.-N. 
Keywords: Apoptosis
Cadmium
CD4+/CD8+
Development
Susceptibility
Thymocyte
Issue Date: 2001
Citation: Dong, S., Shen, H.-M., Ong, C.-N. (2001). Cadmium-induced apoptosis and phenotypic changes in mouse thymocytes. Molecular and Cellular Biochemistry 222 (1-2) : 11-20. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1017970030131
Abstract: At present cadmium (Cd)-induced immunotoxicity and the mechanisms involved have not been fully elucidated. The main objective of the present study is to explore the apoptogenic property of Cd in primary cultured mouse thymocytes and its effect on cell surface marker expression and phenotypic changes. Cd-induced thymocyte apoptosis was determined by TdT-mediated dUTP nick end labeling (TUNEL) assay, DNA content/cell cycle analysis and DNA gel electrophoresis. The results showed that Cd was able to cause apoptosis in mouse thymocytes in a time- and dose-dependent manner. Moreover, different subsets of thymocytes possessed different susceptibility to the apoptotic effect of Cd, in the order of CD8+ > CD4 CD8 (double negative cells, DN) > CD4+CD8+ (double positive cells, DP) > CD4+. Cd treatment also altered thymocyte surface marker expression, leading to evident phenotypic changes. Such changes were characterized by a decline in DP cells and a marked decrease in CD4+/CD8+ ratio, mainly due to a significant increase in CD8+ subsets. These observations help to obtain a better understanding of the immunotoxic and immunomodulatory effects of Cd.
Source Title: Molecular and Cellular Biochemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/113388
ISSN: 03008177
DOI: 10.1023/A:1017970030131
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