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Title: Molecular identification and characterization of the Arabidopsis AtADF1, AtADF5 and AtADF6 genes
Authors: Dong, C.-H. 
Kost, B.
Xia, G.
Chua, N.-H.
Keywords: Actin cytoskeleton
ADF (actin depolymerizing factor)
Arabidopsis thaliana
Issue Date: 2001
Citation: Dong, C.-H., Kost, B., Xia, G., Chua, N.-H. (2001). Molecular identification and characterization of the Arabidopsis AtADF1, AtADF5 and AtADF6 genes. Plant Molecular Biology 45 (5) : 517-527. ScholarBank@NUS Repository.
Abstract: Actin depolymerizing factor (ADF) is a key regulator of the organization of the actin cytoskeleton during various cellular activities. We found that ADF genes in Arabidopsis form a large family consisting of at least nine members, four of which were cloned and sequenced in this study. Comparison of genomic and cDNA sequences showed that the AtADF1, AtADF5, and AtADF6 genes all contain two introns at conserved positions. Analysis of transgenic Arabidopsis plants carrying promoter-GUS fusion constructs revealed that AtADF1 and AtADF6 are expressed in the vascular tissues of all organs, whereas expression of AtADF5 is restricted to the root tip meristem. GFP-AtADF1, GFP-AtADF5, and GFP-AtADF6 fusion proteins were found to bind to actin filaments in vivo, and to reorganize the actin cytoskeleton when transiently expressed in plant cells.
Source Title: Plant Molecular Biology
ISSN: 01674412
DOI: 10.1023/A:1010687911374
Appears in Collections:Staff Publications

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