Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1010687911374
Title: Molecular identification and characterization of the Arabidopsis AtADF1, AtADF5 and AtADF6 genes
Authors: Dong, C.-H. 
Kost, B.
Xia, G.
Chua, N.-H.
Keywords: Actin cytoskeleton
ADF (actin depolymerizing factor)
Arabidopsis thaliana
GFP
GUS
Issue Date: 2001
Citation: Dong, C.-H., Kost, B., Xia, G., Chua, N.-H. (2001). Molecular identification and characterization of the Arabidopsis AtADF1, AtADF5 and AtADF6 genes. Plant Molecular Biology 45 (5) : 517-527. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1010687911374
Abstract: Actin depolymerizing factor (ADF) is a key regulator of the organization of the actin cytoskeleton during various cellular activities. We found that ADF genes in Arabidopsis form a large family consisting of at least nine members, four of which were cloned and sequenced in this study. Comparison of genomic and cDNA sequences showed that the AtADF1, AtADF5, and AtADF6 genes all contain two introns at conserved positions. Analysis of transgenic Arabidopsis plants carrying promoter-GUS fusion constructs revealed that AtADF1 and AtADF6 are expressed in the vascular tissues of all organs, whereas expression of AtADF5 is restricted to the root tip meristem. GFP-AtADF1, GFP-AtADF5, and GFP-AtADF6 fusion proteins were found to bind to actin filaments in vivo, and to reorganize the actin cytoskeleton when transiently expressed in plant cells.
Source Title: Plant Molecular Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/112934
ISSN: 01674412
DOI: 10.1023/A:1010687911374
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

54
checked on Dec 5, 2018

WEB OF SCIENCETM
Citations

47
checked on Nov 20, 2018

Page view(s)

24
checked on Oct 12, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.