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|Title:||Vitrification of encapsulated hepatocytes with reduced cooling/warming rates||Authors:||Kuleshova, L.L.
|Issue Date:||Jul-2004||Citation:||Kuleshova, L.L.,Wang, X.W.,Wu, Y.N.,Zhou, Y.,Yu, H. (2004-07). Vitrification of encapsulated hepatocytes with reduced cooling/warming rates. Cryo-Letters 25 (4) : 241-254. ScholarBank@NUS Repository.||Abstract:||We have used microencapsulated hepatocytes as model to develop a method of vitreous cryopreservation of large quantities of cell-containing constructs. The method included a pre-equilibration procedure in which the amount of penetrating cryoprotectant was gradually increased by 15% in each step. The optimal vitrification solution consists of 40% ethylene glycol and 0.6M sucrose. The concentration of 1M sucrose used for the first dilution solution with subsequent decrease of sucrose concentration to 0.7 M sucrose and by 0.2-0.15M for each subsequent step. This sucrose dilution procedure had no adverse effect on cell functions. Three cooling rates (400°C/min and above) and three warming rates (650°C/min and above), in combination with the proposed vitrification solution, were equally effective. The optimization of the procedure and solutions allow microencapsulated hepatocytes to be preserved with almost 100% retention of cell functions and no detectable damage to the fragile microcapsules. The de-linking of the cooling/warming rates with the effectiveness of vitrification potentially paves the way for large scale cryopreservation of complex tissue engineered constructs.||Source Title:||Cryo-Letters||URI:||http://scholarbank.nus.edu.sg/handle/10635/112817||ISSN:||01432044|
|Appears in Collections:||Staff Publications|
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