Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/112817
Title: Vitrification of encapsulated hepatocytes with reduced cooling/warming rates
Authors: Kuleshova, L.L.
Wang, X.W.
Wu, Y.N. 
Zhou, Y.
Yu, H. 
Keywords: Cryopreservation
Double straw
Encapsulated hepatocytes
Ethylene glycol
Vitrification
Issue Date: Jul-2004
Citation: Kuleshova, L.L.,Wang, X.W.,Wu, Y.N.,Zhou, Y.,Yu, H. (2004-07). Vitrification of encapsulated hepatocytes with reduced cooling/warming rates. Cryo-Letters 25 (4) : 241-254. ScholarBank@NUS Repository.
Abstract: We have used microencapsulated hepatocytes as model to develop a method of vitreous cryopreservation of large quantities of cell-containing constructs. The method included a pre-equilibration procedure in which the amount of penetrating cryoprotectant was gradually increased by 15% in each step. The optimal vitrification solution consists of 40% ethylene glycol and 0.6M sucrose. The concentration of 1M sucrose used for the first dilution solution with subsequent decrease of sucrose concentration to 0.7 M sucrose and by 0.2-0.15M for each subsequent step. This sucrose dilution procedure had no adverse effect on cell functions. Three cooling rates (400°C/min and above) and three warming rates (650°C/min and above), in combination with the proposed vitrification solution, were equally effective. The optimization of the procedure and solutions allow microencapsulated hepatocytes to be preserved with almost 100% retention of cell functions and no detectable damage to the fragile microcapsules. The de-linking of the cooling/warming rates with the effectiveness of vitrification potentially paves the way for large scale cryopreservation of complex tissue engineered constructs.
Source Title: Cryo-Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/112817
ISSN: 01432044
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

53
checked on Aug 1, 2021

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.