Please use this identifier to cite or link to this item:
Title: Tethered spheroids as an invitro hepatocyte model for drug safety screening
Authors: Xia, L.
Sakban, R.B.
Qu, Y.
Hong, X.
Zhang, W. 
Nugraha, B.
Tong, W.H.
Ananthanarayanan, A.
Zheng, B.
Chau, I.Y.Y.
Jia, R.
McMillian, M.
Silva, J.
Dallas, S.
Yu, H. 
Keywords: 3D hepatocyte model
CYP450 enzymes
Drug safety testing
Issue Date: Mar-2012
Citation: Xia, L., Sakban, R.B., Qu, Y., Hong, X., Zhang, W., Nugraha, B., Tong, W.H., Ananthanarayanan, A., Zheng, B., Chau, I.Y.Y., Jia, R., McMillian, M., Silva, J., Dallas, S., Yu, H. (2012-03). Tethered spheroids as an invitro hepatocyte model for drug safety screening. Biomaterials 33 (7) : 2165-2176. ScholarBank@NUS Repository.
Abstract: Hepatocyte spheroids mimic many invivo liver-tissue phenotypes but increase in size during extended culture which limits their application in drug testing applications. We have developed an improved hepatocyte 3D spheroid model, namely tethered spheroids, on RGD and galactose-conjugated membranes using an optimized hybrid ratio of the two bioactive ligands. Cells in the spheroid configuration maintained 3D morphology and uncompromised differentiated hepatocyte functions (urea and albumin production), while the spheroid bottom was firmly tethered to the substratum maintaining the spheroid size in multi-well plates. The oblate shape of the tethered spheroids, with an average height of 32μm, ensured efficient nutrient, oxygen and drug access to all the cells within the spheroid structure. Cytochrome P450 induction by prototypical inducers was demonstrated in the tethered spheroids and was comparable or better than that observed with hepatocyte sandwich cultures. These data suggested that tethered 3D hepatocyte spheroids may be an excellent alternative to 2D hepatocyte culture models for drug safety applications. © 2011 Elsevier Ltd.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2011.12.006
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Nov 15, 2019


checked on Nov 15, 2019

Page view(s)

checked on Nov 8, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.