Please use this identifier to cite or link to this item:
Title: The transmembrane domain of N-glucosaminyltransferase I contains a Golgi retention signal
Authors: Tang, B.L. 
Wong, S.H. 
Low, S.H. 
Hong, W. 
Issue Date: 15-May-1992
Citation: Tang, B.L.,Wong, S.H.,Low, S.H.,Hong, W. (1992-05-15). The transmembrane domain of N-glucosaminyltransferase I contains a Golgi retention signal. Journal of Biological Chemistry 267 (14) : 10122-10126. ScholarBank@NUS Repository.
Abstract: The enzyme N-acetylglucosaminyltransferase I (NT, EC is a resident type II transmembrane protein of the Golgi apparatus. To delineate the portion of its primary sequence that is responsible for the Golgi retention of this protein, we constructed chimeras containing different N-terminal portions of NT joined to a reporter sequence, the ectodomain of a type II surface membrane protein. These chimeric proteins were found to be retained in the Golgi apparatus as assessed by cell surface biotinylation and immunofluorescence. We found that the transmembrane domain of NT is sufficient to confer Golgi retention of the fusion proteins and propose that it contains the Golgi retention signal of the parent molecule.
Source Title: Journal of Biological Chemistry
ISSN: 00219258
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Feb 2, 2023

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.