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| Title: | Primer extension. | Authors: | Smith, D.R. | Issue Date: | 1993 | Citation: | Smith, D.R. (1993). Primer extension.. Methods in molecular biology (Clifton, N.J.) 18 : 373-378. ScholarBank@NUS Repository. | Abstract: | Primer extension can be viewed as a technique complementary to S1 nuclease mapping (see Chapter 43). In Sl nuclease mapping analysis, a DNA:RNA hybrid is resected back to the point of divergence between the RNA and the DNA. In primer extension, a DNA probe is annealed to an RNA template and then extended in a 3';- 5'; direction to the start of the RNA molecule. The most common usages for this technique are in determining the size of full-length RNA transcripts and mapping transcription initiation sites. | Source Title: | Methods in molecular biology (Clifton, N.J.) | URI: | http://scholarbank.nus.edu.sg/handle/10635/112021 | ISSN: | 19406029 |
| Appears in Collections: | Staff Publications |
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