Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/112021
| DC Field | Value | |
|---|---|---|
| dc.title | Primer extension. | |
| dc.contributor.author | Smith, D.R. | |
| dc.date.accessioned | 2014-11-28T02:52:16Z | |
| dc.date.available | 2014-11-28T02:52:16Z | |
| dc.date.issued | 1993 | |
| dc.identifier.citation | Smith, D.R. (1993). Primer extension.. Methods in molecular biology (Clifton, N.J.) 18 : 373-378. ScholarBank@NUS Repository. | |
| dc.identifier.issn | 19406029 | |
| dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/112021 | |
| dc.description.abstract | Primer extension can be viewed as a technique complementary to S1 nuclease mapping (see Chapter 43). In Sl nuclease mapping analysis, a DNA:RNA hybrid is resected back to the point of divergence between the RNA and the DNA. In primer extension, a DNA probe is annealed to an RNA template and then extended in a 3';- 5'; direction to the start of the RNA molecule. The most common usages for this technique are in determining the size of full-length RNA transcripts and mapping transcription initiation sites. | |
| dc.source | Scopus | |
| dc.type | Article | |
| dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
| dc.description.sourcetitle | Methods in molecular biology (Clifton, N.J.) | |
| dc.description.volume | 18 | |
| dc.description.page | 373-378 | |
| dc.identifier.isiut | NOT_IN_WOS | |
| Appears in Collections: | Staff Publications | |
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