GS28, a 28-kilodalton golgi SNARE that participates in ER-Golgi transport

Abstract

Little is known about the integral membrane proteins that participate in the early secretory pathway of mammalian cells, The complementary DNA encoding a 28-kilodalton protein (p28) of the cis-Golgi was cloned and sequenced. The protein was predicted to contain a central coiled-coil domain with a carboxyl-terminal membrane anchor. An in vitro assay for endoplasmic reticulum-Golgi transport was used to show that p28 participates in the docking and fusion stage of this transport event. Biochemical studies established that p28 is a core component of the Golgi SNAP receptor (SNARE) complex.