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Title: Gene from tropical Bacillus sphaericus encoding a protease closely related to subtilisins from Antarctic bacilli
Authors: Wati, M.R. 
Thanabalu, T. 
Porter, A.G. 
Keywords: Bacillus sphaericus
mosquitocidal toxin
Issue Date: 2-May-1997
Citation: Wati, M.R., Thanabalu, T., Porter, A.G. (1997-05-02). Gene from tropical Bacillus sphaericus encoding a protease closely related to subtilisins from Antarctic bacilli. Biochimica et Biophysica Acta - Gene Structure and Expression 1352 (1) : 56-62. ScholarBank@NUS Repository.
Abstract: We undertook to identify the protease(s) involved in the in vivo degradation of the 100 kDa mosquitocidal toxin (Mtx) from Bacillus sphaericus SSII-1 and isolated a B. sphaericus SSII-1 gene flanked upstream by a typical Shine-Dalgarno ribosome binding site and downstream by a strong ρ-independent transcription terminator. The predicted ORF encodes a 432 amino acid protein with significant homology throughout its sequence to two subtilisin-like serine proteases from the Antarctic psychrophilic (cold-adapted) bacilli, TA39 and TA41. The predicted N-terminal sequence suggests that the B. sphaericus protease is related to sfericase, a partially characterized serine protease from B. sphaericus. Only B. sphaericus strains which produce Mtx-degrading protease activity harbour the subtilisin-like protease gene, suggesting that this protease may be responsible for or contribute to the degradation of Mtx in B. sphaericus SSII-1. A 36-kDa protease with Mtx-degrading activity and similar properties to sfericase was also purified from sporulated cultures of B. sphaericus SSII-1. Further studies are needed to determine the relationship of this protease to sfericase and to the predicted product of the subtilisin-like serine protease gene.
Source Title: Biochimica et Biophysica Acta - Gene Structure and Expression
ISSN: 01674781
DOI: 10.1016/S0167-4781(97)00023-7
Appears in Collections:Staff Publications

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