Please use this identifier to cite or link to this item:
https://doi.org/10.1016/S0167-4781(97)00023-7
DC Field | Value | |
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dc.title | Gene from tropical Bacillus sphaericus encoding a protease closely related to subtilisins from Antarctic bacilli | |
dc.contributor.author | Wati, M.R. | |
dc.contributor.author | Thanabalu, T. | |
dc.contributor.author | Porter, A.G. | |
dc.date.accessioned | 2014-11-28T02:50:53Z | |
dc.date.available | 2014-11-28T02:50:53Z | |
dc.date.issued | 1997-05-02 | |
dc.identifier.citation | Wati, M.R., Thanabalu, T., Porter, A.G. (1997-05-02). Gene from tropical Bacillus sphaericus encoding a protease closely related to subtilisins from Antarctic bacilli. Biochimica et Biophysica Acta - Gene Structure and Expression 1352 (1) : 56-62. ScholarBank@NUS Repository. https://doi.org/10.1016/S0167-4781(97)00023-7 | |
dc.identifier.issn | 01674781 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/111897 | |
dc.description.abstract | We undertook to identify the protease(s) involved in the in vivo degradation of the 100 kDa mosquitocidal toxin (Mtx) from Bacillus sphaericus SSII-1 and isolated a B. sphaericus SSII-1 gene flanked upstream by a typical Shine-Dalgarno ribosome binding site and downstream by a strong ρ-independent transcription terminator. The predicted ORF encodes a 432 amino acid protein with significant homology throughout its sequence to two subtilisin-like serine proteases from the Antarctic psychrophilic (cold-adapted) bacilli, TA39 and TA41. The predicted N-terminal sequence suggests that the B. sphaericus protease is related to sfericase, a partially characterized serine protease from B. sphaericus. Only B. sphaericus strains which produce Mtx-degrading protease activity harbour the subtilisin-like protease gene, suggesting that this protease may be responsible for or contribute to the degradation of Mtx in B. sphaericus SSII-1. A 36-kDa protease with Mtx-degrading activity and similar properties to sfericase was also purified from sporulated cultures of B. sphaericus SSII-1. Further studies are needed to determine the relationship of this protease to sfericase and to the predicted product of the subtilisin-like serine protease gene. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0167-4781(97)00023-7 | |
dc.source | Scopus | |
dc.subject | Bacillus sphaericus | |
dc.subject | mosquitocidal toxin | |
dc.subject | protease | |
dc.type | Article | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.description.doi | 10.1016/S0167-4781(97)00023-7 | |
dc.description.sourcetitle | Biochimica et Biophysica Acta - Gene Structure and Expression | |
dc.description.volume | 1352 | |
dc.description.issue | 1 | |
dc.description.page | 56-62 | |
dc.description.coden | BBGSD | |
dc.identifier.isiut | A1997WY05500009 | |
Appears in Collections: | Staff Publications |
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