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Title: Direct PCR amplification and sequence analysis of extrachromosomal Plasmodium DNA from dried blood spots
Authors: Tan, T.M.C. 
Nelson, J.S. 
Ng, H.C. 
Ting, R.C.Y. 
Kara, U.A.K. 
Keywords: Blood spots
Extrachromosomal plastid
PCR amplification
Issue Date: 1997
Citation: Tan, T.M.C., Nelson, J.S., Ng, H.C., Ting, R.C.Y., Kara, U.A.K. (1997). Direct PCR amplification and sequence analysis of extrachromosomal Plasmodium DNA from dried blood spots. Acta Tropica 68 (1) : 105-114. ScholarBank@NUS Repository.
Abstract: The Plasmodium parasite possesses two extrachromosomal genomes; the mitochondrial genetic element and the extrachromosomal plastid-like DNA. The latter has only been fully described for one culture strain of P. falciparum. In this study, a rapid procedure for amplifying plastid DNA from dried blood spots of blood infected with different malaria species was developed. PCR amplification of a 595 bp fragment within the plastid-like large subunit ribosomal-RNA (LSU-rRNA) gene was achieved using primers derived from the P. falciparum sequence. The PCR product was observed in all Plasmodium species examined. Sequence analysis of amplified products homologous to an LSU-rRNA fragment of the plastid-like extrachromosomal circle revealed extensive conservation between Plasmodium species including P. falciparum, P. vivax, P. malariae and P. berghei.
Source Title: Acta Tropica
ISSN: 0001706X
DOI: 10.1016/S0001-706X(97)00080-6
Appears in Collections:Staff Publications

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