Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0001-706X(97)00080-6
DC FieldValue
dc.titleDirect PCR amplification and sequence analysis of extrachromosomal Plasmodium DNA from dried blood spots
dc.contributor.authorTan, T.M.C.
dc.contributor.authorNelson, J.S.
dc.contributor.authorNg, H.C.
dc.contributor.authorTing, R.C.Y.
dc.contributor.authorKara, U.A.K.
dc.date.accessioned2014-11-28T02:50:25Z
dc.date.available2014-11-28T02:50:25Z
dc.date.issued1997
dc.identifier.citationTan, T.M.C., Nelson, J.S., Ng, H.C., Ting, R.C.Y., Kara, U.A.K. (1997). Direct PCR amplification and sequence analysis of extrachromosomal Plasmodium DNA from dried blood spots. Acta Tropica 68 (1) : 105-114. ScholarBank@NUS Repository. https://doi.org/10.1016/S0001-706X(97)00080-6
dc.identifier.issn0001706X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/111854
dc.description.abstractThe Plasmodium parasite possesses two extrachromosomal genomes; the mitochondrial genetic element and the extrachromosomal plastid-like DNA. The latter has only been fully described for one culture strain of P. falciparum. In this study, a rapid procedure for amplifying plastid DNA from dried blood spots of blood infected with different malaria species was developed. PCR amplification of a 595 bp fragment within the plastid-like large subunit ribosomal-RNA (LSU-rRNA) gene was achieved using primers derived from the P. falciparum sequence. The PCR product was observed in all Plasmodium species examined. Sequence analysis of amplified products homologous to an LSU-rRNA fragment of the plastid-like extrachromosomal circle revealed extensive conservation between Plasmodium species including P. falciparum, P. vivax, P. malariae and P. berghei.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0001-706X(97)00080-6
dc.sourceScopus
dc.subjectBlood spots
dc.subjectExtrachromosomal plastid
dc.subjectMalaria
dc.subjectPCR amplification
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.doi10.1016/S0001-706X(97)00080-6
dc.description.sourcetitleActa Tropica
dc.description.volume68
dc.description.issue1
dc.description.page105-114
dc.description.codenACTRA
dc.identifier.isiutA1997YC21100009
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