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|Title:||Graft-versus-host reaction (GVHR) in clonal amago salmon, Oncorhynchus rhodurus||Authors:||Qin, Q.W.
Clonal amago salmon
Graft-versus-host disease (GVHD)
Graft-versus-host reaction (GVHR)
|Issue Date:||8-Oct-2002||Citation:||Qin, Q.W., Ototake, M., Nagoya, H., Nakanishi, T. (2002-10-08). Graft-versus-host reaction (GVHR) in clonal amago salmon, Oncorhynchus rhodurus. Veterinary Immunology and Immunopathology 89 (1-2) : 83-89. ScholarBank@NUS Repository. https://doi.org/10.1016/S0165-2427(02)00187-3||Abstract:||The graft-versus-host reaction (GVHR) was demonstrated in a salmonid model system of clonal diploid and triploid amago salmon. Triploid operculum grafts on clonal diploid evoked an acute rejection within 12 days. Grafts exchanged among triploid amago salmon exhibited prolonged survival for 18 days. In contrast, diploid grafts on triploid, and allografts among clonal diploid amago salmon were accepted. A typical GVHR was induced in triploid recipients by intraperitonal injection of head kidney cells from sensitised diploid donors. The clinical signs of graft-versus-host disease (GVHD) were observed in the recipients after 1 week of cell injection as a loss of appetite and appearance of solid faeces, followed by haemorrhage, local swelling of ventral skin and an enlarged spleen. Three of six fish died within 1 month. Water temperature and frequency of sensitisation are critical to induce GVHR. Diploid donors had to be sensitised three times at 20°C to induce the typical GVHR. GVHR was most effectively induced by head kidney cells, followed by peripheral blood leucocytes (PBL) and spleen cells. Ploidy analysis by flow cytometry revealed that the donor head kidney cells greatly increased in the recipient liver, head kidney and spleen, and reached the peak after 9 days of donor cell injection. The results in the present study are quite similar to the findings in ginbuna and ginbuna-gold fish hybrid system, suggesting the presence of T cells in salmonid as well as cyprinid fish. © 2002 Elsevier Science B.V. All rights reserved.||Source Title:||Veterinary Immunology and Immunopathology||URI:||http://scholarbank.nus.edu.sg/handle/10635/110869||ISSN:||01652427||DOI:||10.1016/S0165-2427(02)00187-3|
|Appears in Collections:||Staff Publications|
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