Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jviromet.2004.12.005
Title: Development of a flow cytometry based method for rapid and sensitive detection of a novel marine fish iridovirus in cell culture
Authors: Qin, Q.W. 
Gin, K.Y.-H.
Lee, L.Y.
Gedaria, A.I. 
Zhang, S.
Keywords: Cell culture
Flow cytometry
Immunofluorescence
Marine fish iridovirus (SGIV)
Monoclonal antibody
Issue Date: Apr-2005
Citation: Qin, Q.W., Gin, K.Y.-H., Lee, L.Y., Gedaria, A.I., Zhang, S. (2005-04). Development of a flow cytometry based method for rapid and sensitive detection of a novel marine fish iridovirus in cell culture. Journal of Virological Methods 125 (1) : 49-54. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jviromet.2004.12.005
Abstract: A sensitive and accurate flow cytometry (FCM) based method has been developed to detect and quantitate a novel marine fish iridovirus (Singapore grouper iridovirus, SGIV) after amplification in cell cultures. Confluent grouper cell (GP) monolayers were infected with SGIV. When advanced cytopathic effect (CPE) appeared, the cell cultures were fixed and permeabilized, and then reacted with monoclonal antibodies specific against SGIV, followed by a second antibody conjugated with FITC (anti-mouse IgG-FITC). A Coulter® EPICS Elite ESP flow cytometer was used to directly detect and analyze the percentage of virus-infected cells. Three fixation and permeabilization methods were evaluated. The kinetics of the virus infection process was determined. The FCM procedure enables large amounts of cells to be screened rapidly for infectivity, and it can also detect low levels of virus infection. As early as 8 h after inoculation with the virus, 0.34% of infected cells were detected in cell culture. The maximum level of infection was obtained at 72 h. The efficiency and reliability of the FCM procedure were compared with those of the standard methods of immunofluorescence microscopy and PCR. © 2004 Elsevier B.V. All rights reserved.
Source Title: Journal of Virological Methods
URI: http://scholarbank.nus.edu.sg/handle/10635/110851
ISSN: 01660934
DOI: 10.1016/j.jviromet.2004.12.005
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

16
checked on Jan 26, 2023

WEB OF SCIENCETM
Citations

12
checked on Jan 26, 2023

Page view(s)

174
checked on Jan 26, 2023

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.