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Title: NRAMP1 and hGPX1 gene polymorphism and response to bacillus Calmette-Guérin therapy for bladder cancer
Authors: Chiong, E.
Kesavan, A.
Mahendran, R. 
Chan, Y.H.
Sng, J.H. 
Lim, Y.K.
Kamaraj, R.
Tan, T.M.C. 
Esuvaranathan, K.
Keywords: Bacillus Calmette-Guérin
Bladder cancer
Gene polymorphism
Issue Date: Mar-2011
Citation: Chiong, E., Kesavan, A., Mahendran, R., Chan, Y.H., Sng, J.H., Lim, Y.K., Kamaraj, R., Tan, T.M.C., Esuvaranathan, K. (2011-03). NRAMP1 and hGPX1 gene polymorphism and response to bacillus Calmette-Guérin therapy for bladder cancer. European Urology 59 (3) : 430-437. ScholarBank@NUS Repository.
Abstract: Background: The natural resistance-associated macrophage protein 1 (NRAMP1) gene is associated with susceptibility to Mycobacterium tuberculosis in humans and to bacillus Calmette-Guérin (BCG) in mice. The detoxification enzyme, human glutathione peroxidase 1 (hGPX1), is associated with recurrence of bladder cancer (BCa). Objective: To determine whether NRAMP1 and hGPX1 gene polymorphisms correlate with response to BCG immunotherapy for non-muscle-invasive BCa (NMIBC). Design, setting, and participants: DNA was obtained from the peripheral blood of 99 NMIBC patients who were prospectively randomized to receive postresection intravesical BCG (81 mg [n = 50] or 27 mg [n = 19]) or BCG (27 mg) with interferon alpha (IFN-α; n = 30). The median follow-up time was 60 mo. Intervention: Intravesical BCG or BCG-IFN-α. Measurements: Restriction fragment length polymorphism (RFLP) analysis was performed to identify polymorphisms in the NRAMP1 promoter region (GT repeat number) and at position 543 (aspartate [D] and/or asparagine [N] expression) within the NRAMP1 protein (D543N) and position 198 (proline and/or leucine expression) within the hGPX1 protein (Pro198Leu). Data were analyzed using χ2 analysis, multivariate analysis, and Kaplan-Meier curves. Results and limitations: On univariate analysis, the NRAMP1 D543N G:G genotype had decreased cancer-specific survival (CSS; p = 0.036). The hGPX1 CT genotype (Pro-Leu) had decreased recurrence time (p = 0.03) after BCG therapy. On multivariate analysis, patients with the NRAMP1 D543N G:G genotype and allele 3 (GT)n polymorphism had decreased recurrence time (p = 0.014 and p = 0.03) after BCG therapy. The limitation of this study was its small sample size. Conclusions: Polymorphisms of the NRAMP1 and hGPX1 genes may be associated with recurrence of BCa after BCG immunotherapy. © 2010 European Association of Urology. Published by Elsevier B.V. All rights reserved.
Source Title: European Urology
ISSN: 03022838
DOI: 10.1016/j.eururo.2010.11.031
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