Please use this identifier to cite or link to this item: https://doi.org/10.1111/iej.12185
Title: Fusobacterium nucleatum induces cytokine production through Toll-like-receptor-independent mechanism
Authors: Quah, S.Y.
Bergenholtz, G.
Tan, K.S. 
Keywords: Bacterial invasion
Fusobacterium nucleatum
Nucleotide-binding oligomerization domain-1
Nucleotide-binding oligomerization domain-2
P38 mitogen-activated protein kinase
Toll-like receptors
Issue Date: 2014
Citation: Quah, S.Y., Bergenholtz, G., Tan, K.S. (2014). Fusobacterium nucleatum induces cytokine production through Toll-like-receptor-independent mechanism. International Endodontic Journal 47 (6) : 550-559. ScholarBank@NUS Repository. https://doi.org/10.1111/iej.12185
Abstract: Aim: To determine whether Fusobacterium nucleatum's ability to invade cells allows the bacteria to activate pro-inflammatory response through cytosolic pattern recognition receptors, independent of surface Toll-like receptors (TLRs). Methodology: HEK293T cells, which lack endogenous TLRs, and overexpressing dominant negative myeloid differentiation primary response gene 88 (MyD88DN) protein, were infected with F. nucleatum and the production of interleukin-8 (IL-8) was determined. The necessity for intracellular invasion of the bacteria for cytokine production was also investigated by blocking bacterial invasion with cytochalasin D. The roles of NFK{green}B and p38 mitogen-activated protein kinase (MAPK) and nucleotide-binding oligomerization domain-1 (NOD-1) signalling pathways in F. nucleatum-induced IL-8 secretion were determined. Results: Fusobacterium nucleatum-infected HEK293T cells produced IL-8 independent of the MYD88 signalling. This response was inhibited by preventing F. nucleatum invasion into HEK293T cells. p38 MAPK but not the NFK{green}B signalling pathway was required for F. nucleatum-mediated IL-8 production. HEK293T cells expressed NOD-1 but not NOD-2. Yet, inhibition of NOD-1 signalling did not affect F. nucleatum-induced IL-8 secretion. Conclusions: Fusobacterium nucleatum invasion led to cytokine production, which is mediated by the p38 MAPK signalling but independent of TLRs, NOD-1, NOD-2 and NFKB signalling. © 2014 by The American College of Veterinary Surgeons.
Source Title: International Endodontic Journal
URI: http://scholarbank.nus.edu.sg/handle/10635/109358
ISSN: 13652591
DOI: 10.1111/iej.12185
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