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|Title:||Fusobacterium nucleatum activates the immune response through retinoic acid-inducible gene i||Authors:||Lee, P.
nuclear factor kappa B
pattern recognition receptor
periodontal ligament fibroblast
|Issue Date:||Feb-2014||Citation:||Lee, P., Tan, K.S. (2014-02). Fusobacterium nucleatum activates the immune response through retinoic acid-inducible gene i. Journal of Dental Research 93 (2) : 162-168. ScholarBank@NUS Repository. https://doi.org/10.1177/0022034513516346||Abstract:||Retinoic acid-inducible gene I (RIG-I) is a cytosolic pattern recognition receptor involved in the sensing of RNA viruses and the initiation of antiviral responses. Fusobacterium nucleatum, a Gram-negative anaerobic bacterium associated with periodontal disease, is capable of invading cells. We hypothesized that F. nucleatum's ability to invade cells allows the microorganism to activate the immune response through RIG-I. Bacterial invasion was found to be necessary for F. nucleatum-induced nuclear factor kappa B (NF-κB) activation. Following invasion of the human periodontal ligament fibroblast (PDLF), F. nucleatum was located in the cytosol. F. nucleatum infection led to an 80-fold increase in RIG-I expression. Silencing RIG-I in PDLF by siRNA led to a significant decrease of NF-κB activation and expression of proinflammatory genes. Additionally, F. nucleatum was able to secrete nucleic acids, and introduction of F. nucleatum RNA into PDLF led to a RIG-I-dependent activation of NF-κB. Our findings showed RIG-I to be involved in the recognition of F. nucleatum. The function of RIG-I is likely to be broad and not limited to sensing of viruses only. Hence, this receptor may play an important role in detecting invasive forms of oral pathogens and contribute to inflammation in periodontal tissues. © International & American Associations for Dental Research.||Source Title:||Journal of Dental Research||URI:||http://scholarbank.nus.edu.sg/handle/10635/109357||ISSN:||00220345||DOI:||10.1177/0022034513516346|
|Appears in Collections:||Staff Publications|
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