Please use this identifier to cite or link to this item:
|Title:||A global transcriptomic view of the multifaceted role of glutathione peroxidase-1 in cerebral ischemic-reperfusion injury||Authors:||Chen, M.J.
Glutathione peroxidase 1
Nuclear factor (erythroid-related 2)-like 2
|Issue Date:||15-Mar-2011||Citation:||Chen, M.J., Wong, C.H.Y., Peng, Z.F., Manikandan, J., Melendez, A.J., Tan, T.M., Crack, P.J., Cheung, N.S. (2011-03-15). A global transcriptomic view of the multifaceted role of glutathione peroxidase-1 in cerebral ischemic-reperfusion injury. Free Radical Biology and Medicine 50 (6) : 736-748. ScholarBank@NUS Repository. https://doi.org/10.1016/j.freeradbiomed.2010.12.025||Abstract:||Transient cerebral ischemia often results in secondary ischemic/reperfusion injury, the pathogenesis of which remains unclear. This study provides a comprehensive, temporal description of the molecular events contributing to neuronal injury after transient cerebral ischemia. Intraluminal middle cerebral artery occlusion (MCAO) was performed to induce a 2-h ischemia with reperfusion. Microarray analysis was then performed on the infarct cortex of wild-type (WT) and glutathione peroxidase-1 (a major antioxidant enzyme) knockout (Gpx1 -/-) mice at 8 and 24 h postreperfusion to identify differential gene expression profile patterns and potential alternative injury cascades in the absence of Gpx1, a crucial antioxidant enzyme, in cerebral ischemia. Genes with at least ± 1.5-fold change in expression at either time point were considered significant. Global transcriptomic analyses demonstrated that 70% of the WT-MCAO profile overlapped with that of Gpx1-/--MCAO, and 28% vice versa. Critical analysis of the 1034 gene probes specific to the Gpx1 -/--MCAO profile revealed regulation of additional novel pathways, including the p53-mediated proapoptotic pathway and Fas ligand (CD95/Apo1)-mediated pathways; downplay of the Nrf2 antioxidative cascade; and ubiquitin-proteasome system dysfunction. Therefore, this comparative study forms the foundation for the establishment of screening platforms for target definition in acute cerebral ischemia intervention. © 2010 Elsevier Inc. All rights reserved.||Source Title:||Free Radical Biology and Medicine||URI:||http://scholarbank.nus.edu.sg/handle/10635/109143||ISSN:||08915849||DOI:||10.1016/j.freeradbiomed.2010.12.025|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jun 9, 2021
WEB OF SCIENCETM
checked on Jun 9, 2021
checked on Jun 7, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.