Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.freeradbiomed.2010.12.025
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dc.title | A global transcriptomic view of the multifaceted role of glutathione peroxidase-1 in cerebral ischemic-reperfusion injury | |
dc.contributor.author | Chen, M.J. | |
dc.contributor.author | Wong, C.H.Y. | |
dc.contributor.author | Peng, Z.F. | |
dc.contributor.author | Manikandan, J. | |
dc.contributor.author | Melendez, A.J. | |
dc.contributor.author | Tan, T.M. | |
dc.contributor.author | Crack, P.J. | |
dc.contributor.author | Cheung, N.S. | |
dc.date.accessioned | 2014-11-26T07:42:22Z | |
dc.date.available | 2014-11-26T07:42:22Z | |
dc.date.issued | 2011-03-15 | |
dc.identifier.citation | Chen, M.J., Wong, C.H.Y., Peng, Z.F., Manikandan, J., Melendez, A.J., Tan, T.M., Crack, P.J., Cheung, N.S. (2011-03-15). A global transcriptomic view of the multifaceted role of glutathione peroxidase-1 in cerebral ischemic-reperfusion injury. Free Radical Biology and Medicine 50 (6) : 736-748. ScholarBank@NUS Repository. https://doi.org/10.1016/j.freeradbiomed.2010.12.025 | |
dc.identifier.issn | 08915849 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/109143 | |
dc.description.abstract | Transient cerebral ischemia often results in secondary ischemic/reperfusion injury, the pathogenesis of which remains unclear. This study provides a comprehensive, temporal description of the molecular events contributing to neuronal injury after transient cerebral ischemia. Intraluminal middle cerebral artery occlusion (MCAO) was performed to induce a 2-h ischemia with reperfusion. Microarray analysis was then performed on the infarct cortex of wild-type (WT) and glutathione peroxidase-1 (a major antioxidant enzyme) knockout (Gpx1 -/-) mice at 8 and 24 h postreperfusion to identify differential gene expression profile patterns and potential alternative injury cascades in the absence of Gpx1, a crucial antioxidant enzyme, in cerebral ischemia. Genes with at least ± 1.5-fold change in expression at either time point were considered significant. Global transcriptomic analyses demonstrated that 70% of the WT-MCAO profile overlapped with that of Gpx1-/--MCAO, and 28% vice versa. Critical analysis of the 1034 gene probes specific to the Gpx1 -/--MCAO profile revealed regulation of additional novel pathways, including the p53-mediated proapoptotic pathway and Fas ligand (CD95/Apo1)-mediated pathways; downplay of the Nrf2 antioxidative cascade; and ubiquitin-proteasome system dysfunction. Therefore, this comparative study forms the foundation for the establishment of screening platforms for target definition in acute cerebral ischemia intervention. © 2010 Elsevier Inc. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.freeradbiomed.2010.12.025 | |
dc.source | Scopus | |
dc.subject | Free radicals | |
dc.subject | Glutathione peroxidase 1 | |
dc.subject | Ischemic stroke | |
dc.subject | Microarray | |
dc.subject | Neurodegeneration | |
dc.subject | Nuclear factor (erythroid-related 2)-like 2 | |
dc.subject | Ubiquitinproteasome pathway | |
dc.type | Article | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1016/j.freeradbiomed.2010.12.025 | |
dc.description.sourcetitle | Free Radical Biology and Medicine | |
dc.description.volume | 50 | |
dc.description.issue | 6 | |
dc.description.page | 736-748 | |
dc.description.coden | FRBME | |
dc.identifier.isiut | 000287770800009 | |
Appears in Collections: | Staff Publications |
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