Please use this identifier to cite or link to this item: https://doi.org/10.1586/14789450.2013.858022
Title: Recent advances in mass spectrometry: Data independent analysis and hyper reaction monitoring
Authors: Law, K.P.
Lim, Y.P. 
Keywords: Aif
Dia
Ft-Arm
Hrm
Mrmhr
Mse
Msx
Prm
Psrm
SAWTH-MS
Issue Date: 2013
Citation: Law, K.P., Lim, Y.P. (2013). Recent advances in mass spectrometry: Data independent analysis and hyper reaction monitoring. Expert Review of Proteomics 10 (6) : 551-566. ScholarBank@NUS Repository. https://doi.org/10.1586/14789450.2013.858022
Abstract: New mass spectrometry (MS) methods, collectively known as data independent analysis and hyper reaction monitoring, have recently emerged. These methods hold promises to address the shortcomings of data-dependent analysis and selected reaction monitoring (SRM) employed in shotgun and targeted proteomics, respectively. They allow MS analyses of all species in a complex sample indiscriminately, or permit SRM-like experiments conducted with full high-resolution product ion spectra, potentially leading to higher sequence coverage or analytical selectivity. These methods include MSE, all-ion fragmentation, Fourier transform-all reaction monitoring, SWATH Acquisition, multiplexed MS/MS, pseudo-SRM (pSRM) and parallel reaction monitoring (PRM). In this review, the strengths and pitfalls of these methods are discussed and illustrated with examples. In essence, the suitability of the use of each method is contingent on the biological questions posed. Although these methods do not fundamentally change the shape of proteomics, they are useful additional tools that should expedite biological discoveries. © 2013 Informa UK, Ltd.
Source Title: Expert Review of Proteomics
URI: http://scholarbank.nus.edu.sg/handle/10635/108682
ISSN: 14789450
DOI: 10.1586/14789450.2013.858022
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

82
checked on Dec 7, 2019

WEB OF SCIENCETM
Citations

68
checked on Nov 28, 2019

Page view(s)

97
checked on Nov 30, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.