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Title: Derivation of Mesenchymal stem cells from human embryonic stem cells
Authors: Choo, A.
Lim, S.K. 
Keywords: CD105
Flow cytometry
Human embryonic stem cells
Mesenchymal stem cells
Issue Date: 2011
Citation: Choo, A.,Lim, S.K. (2011). Derivation of Mesenchymal stem cells from human embryonic stem cells. Methods in Molecular Biology 690 : 175-182. ScholarBank@NUS Repository.
Abstract: Mesenchymal stem cells (MSCs) have been isolated from many tissues including differentiating human embryonic stem cells (hESCs). Derivation of MSCs from hESCs consists of two major steps: differentiation and isolation. In our hands, differentiation of hESCs towards MSC-enriched culture can be induced by trypsinizing hESCs into single cells and plating them on gelatin-coated plates in a culture condition that enhances survival of hESC-derived MSCs and not hESCs. The trypsinized hESCs were grown with feeder support and the medium was supplemented with basic fibroblast growth factor (FGF2) and platelet-derived growth factor (PDGF)-AB. A highly enriched MSC culture could be obtained by repeated passaging by trypsinization. The enriched MSC cultures could be further purified by limiting dilution or FACS sorting for CD105 + or CD73 + and CD24 -. © 2011 Springer Science+Business Media, LLC.
Source Title: Methods in Molecular Biology
ISBN: 9781607619611
ISSN: 10643745
DOI: 10.1007/978-1-60761-962-8_12
Appears in Collections:Staff Publications

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