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https://doi.org/10.1007/978-1-60761-962-8_12
DC Field | Value | |
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dc.title | Derivation of Mesenchymal stem cells from human embryonic stem cells | |
dc.contributor.author | Choo, A. | |
dc.contributor.author | Lim, S.K. | |
dc.date.accessioned | 2014-11-25T09:44:43Z | |
dc.date.available | 2014-11-25T09:44:43Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Choo, A.,Lim, S.K. (2011). Derivation of Mesenchymal stem cells from human embryonic stem cells. Methods in Molecular Biology 690 : 175-182. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/978-1-60761-962-8_12" target="_blank">https://doi.org/10.1007/978-1-60761-962-8_12</a> | |
dc.identifier.isbn | 9781607619611 | |
dc.identifier.issn | 10643745 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/108330 | |
dc.description.abstract | Mesenchymal stem cells (MSCs) have been isolated from many tissues including differentiating human embryonic stem cells (hESCs). Derivation of MSCs from hESCs consists of two major steps: differentiation and isolation. In our hands, differentiation of hESCs towards MSC-enriched culture can be induced by trypsinizing hESCs into single cells and plating them on gelatin-coated plates in a culture condition that enhances survival of hESC-derived MSCs and not hESCs. The trypsinized hESCs were grown with feeder support and the medium was supplemented with basic fibroblast growth factor (FGF2) and platelet-derived growth factor (PDGF)-AB. A highly enriched MSC culture could be obtained by repeated passaging by trypsinization. The enriched MSC cultures could be further purified by limiting dilution or FACS sorting for CD105 + or CD73 + and CD24 -. © 2011 Springer Science+Business Media, LLC. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/978-1-60761-962-8_12 | |
dc.source | Scopus | |
dc.subject | CD105 | |
dc.subject | CD24 | |
dc.subject | CD73 | |
dc.subject | Flow cytometry | |
dc.subject | Human embryonic stem cells | |
dc.subject | Mesenchymal stem cells | |
dc.type | Article | |
dc.contributor.department | SURGERY | |
dc.description.doi | 10.1007/978-1-60761-962-8_12 | |
dc.description.sourcetitle | Methods in Molecular Biology | |
dc.description.volume | 690 | |
dc.description.page | 175-182 | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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