Please use this identifier to cite or link to this item: https://doi.org/10.1021/pr100492x
Title: Cathepsin S mediates gastric cancer cell migration and invasion via a putative network of metastasis-associated proteins
Authors: Yixuan, Y. 
Kiat, L.S. 
Yee, C.L. 
Huiyin, L. 
Yunhao, C. 
Kuan, C.P. 
Hassan, A.
Ting, W.T.
Manuel, S.-T. 
Guan, Y.K.
Pin, L.Y. 
Keywords: cathepsin
CTSS
gastric cancer
invasion
migration
protease
proteomics
secretome
Issue Date: 3-Sep-2010
Citation: Yixuan, Y., Kiat, L.S., Yee, C.L., Huiyin, L., Yunhao, C., Kuan, C.P., Hassan, A., Ting, W.T., Manuel, S.-T., Guan, Y.K., Pin, L.Y. (2010-09-03). Cathepsin S mediates gastric cancer cell migration and invasion via a putative network of metastasis-associated proteins. Journal of Proteome Research 9 (9) : 4767-4778. ScholarBank@NUS Repository. https://doi.org/10.1021/pr100492x
Abstract: Cancer progression is governed by multifaceted interactions of cancer cells with their microenvironment and one of these ways is through secreted compounds. Substances released by gastric cancer cells have not being profiled in a proteome-wide manner. ITRAQ-based tandem mass spectrometry was employed to quantify proteins secreted by HFE145 normal, MKN7 well-differentiated, and MKN45 poorly differentiated gastric cancer cell lines. The expression levels of 237 proteins were found to be significantly different between normal and cancer cells. Further examination of 16 gastric cell lines and 115 clinical samples validated the up-regulation of CTSS expression in gastric cancer. Silencing CTSS expression suppressed the migration and invasion of gastric cancer cells in vitro. Subsequent secretomics revealed that CTSS silencing resulted in changes in expression levels of 197 proteins, one-third of which are implicated in cellular movement. Proteome-wide comparative secretomes of normal and gastric cancer cells were produced that constitute a useful resource for gastric cancer research. CTSS was demonstrated to play novel roles in gastric cancer cell migration and invasion, putatively via a network of proteins associated with cell migration, invasion, or metastasis. Cathepsin S is member of a large group of extracellular proteases, which are attractive drug targets. The implicated role of CTSS in gastric cancer metastasis provides an opportunity to test existing compounds against CTSS for adjuvant therapy and/or treatment of metastatic gastric cancers. © 2010 American Chemical Society.
Source Title: Journal of Proteome Research
URI: http://scholarbank.nus.edu.sg/handle/10635/108285
ISSN: 15353893
DOI: 10.1021/pr100492x
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

50
checked on Jul 16, 2019

WEB OF SCIENCETM
Citations

42
checked on Jul 16, 2019

Page view(s)

101
checked on May 24, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.