Please use this identifier to cite or link to this item:
Title: A novel CARD containing splice-isoform of CIITA regulates nitric oxide synthesis in dendritic cells
Authors: Huang, D.
Lim, S.
Chua, R.Y.R.
Shi, H.
Ng, M.L.
Wong, S.H. 
Keywords: caspase
dendritic cells
nitric oxide synthase
Issue Date: Mar-2010
Citation: Huang, D., Lim, S., Chua, R.Y.R., Shi, H., Ng, M.L., Wong, S.H. (2010-03). A novel CARD containing splice-isoform of CIITA regulates nitric oxide synthesis in dendritic cells. Protein and Cell 1 (3) : 291-306. ScholarBank@NUS Repository.
Abstract: MHC class II expression is controlled mainly at transcriptional level by class II transactivator (CIITA), which is a non-DNA binding coactivator and serves as a master control factor for MHC class II genes expression. Here, we describe the function of a novel splice-isoform of CIITA, DC-expressed caspase inhibitory isoform of CIITA (or DC-CASPIC), and we show that the expression of DCCASPIC in DC is upregulated upon lipopolysaccharides (LPS) induction. DC-CASPIC localizes to mitochondria, and protein-protein interaction study demonstrates that DC-CASPIC interacts with caspases and inhibits its activity in DC. Consistently, DC-CASPIC suppresses caspases-induced degradation of nitric oxide synthase-2 (NOS2) and subsequently promotes the synthesis of nitric oxide (NO). NO is an essential regulatory molecule that modulates the capability of DC in stimulating T cell proliferation/activation in vitro; hence, overexpression of DC-CASPIC in DC enhances this stimulation. Collectively, our findings reveal that DC-CASPIC is a key molecule that regulates caspases activity and NO synthesis in DC. © 2010 Higher Education Press and Springer-Verlag Berlin Heidelberg.
Source Title: Protein and Cell
ISSN: 1674800X
DOI: 10.1007/s13238-010-0039-5
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Jan 20, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.