Please use this identifier to cite or link to this item: https://doi.org/10.1007/s13238-010-0039-5
DC FieldValue
dc.titleA novel CARD containing splice-isoform of CIITA regulates nitric oxide synthesis in dendritic cells
dc.contributor.authorHuang, D.
dc.contributor.authorLim, S.
dc.contributor.authorChua, R.Y.R.
dc.contributor.authorShi, H.
dc.contributor.authorNg, M.L.
dc.contributor.authorWong, S.H.
dc.date.accessioned2014-11-25T09:43:35Z
dc.date.available2014-11-25T09:43:35Z
dc.date.issued2010-03
dc.identifier.citationHuang, D., Lim, S., Chua, R.Y.R., Shi, H., Ng, M.L., Wong, S.H. (2010-03). A novel CARD containing splice-isoform of CIITA regulates nitric oxide synthesis in dendritic cells. Protein and Cell 1 (3) : 291-306. ScholarBank@NUS Repository. https://doi.org/10.1007/s13238-010-0039-5
dc.identifier.issn1674800X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/108235
dc.description.abstractMHC class II expression is controlled mainly at transcriptional level by class II transactivator (CIITA), which is a non-DNA binding coactivator and serves as a master control factor for MHC class II genes expression. Here, we describe the function of a novel splice-isoform of CIITA, DC-expressed caspase inhibitory isoform of CIITA (or DC-CASPIC), and we show that the expression of DCCASPIC in DC is upregulated upon lipopolysaccharides (LPS) induction. DC-CASPIC localizes to mitochondria, and protein-protein interaction study demonstrates that DC-CASPIC interacts with caspases and inhibits its activity in DC. Consistently, DC-CASPIC suppresses caspases-induced degradation of nitric oxide synthase-2 (NOS2) and subsequently promotes the synthesis of nitric oxide (NO). NO is an essential regulatory molecule that modulates the capability of DC in stimulating T cell proliferation/activation in vitro; hence, overexpression of DC-CASPIC in DC enhances this stimulation. Collectively, our findings reveal that DC-CASPIC is a key molecule that regulates caspases activity and NO synthesis in DC. © 2010 Higher Education Press and Springer-Verlag Berlin Heidelberg.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s13238-010-0039-5
dc.sourceScopus
dc.subjectcaspase
dc.subjectCIITA
dc.subjectdendritic cells
dc.subjectnitric oxide synthase
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1007/s13238-010-0039-5
dc.description.sourcetitleProtein and Cell
dc.description.volume1
dc.description.issue3
dc.description.page291-306
dc.identifier.isiut000208510600012
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

197
checked on May 12, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.